Symbiosis™ Pharma is Spark Holland's unique solution for integrated online SPE-LC-MS automation (XLC-MS). The system offers large flexibility in processing different types of samples selecting one of the three fully automated operational modes LC-MS (direct LC without SPE); XLC-MS (online SPE coupled to LC-MS); AMD (advanced method development). The combination of a highly selective online SPE and a superb top line autosampler makes this dedicated high throughput Pharma system the best tool for direct injection of raw biological samples. The zero sample loss injection mode offers the best possible results even when there is little sample volume available (5 µL injection out of a 8 µL sample).