Applikon Biotechnology

Mini Bio Mini Bioreactors

The MiniBio range of bioreactors (250 ml, 500 ml and 1000 ml total volume) is a true scale down of the laboratory scale bioreactors in the 1 to 20 liter range. The MiniBio systems have the same flexibility as the laboratory scale bioreactors. This means that the MiniBio systems can be customized to fit the demands of any process. The small volume reduces medium costs and reduces the use of expensive bench space.

Unique features of small scale bioreactors:

  • Generate more data in less time
  • Easy setup and operation
  • Cultivate using les bench space
  • Generate scalable results
  • Easy data handling


  • Screening studies
  • Media optimization
  • Process optimization
  • Microbial and Cell culture

Configurations for microbial and cell culture bundles

The microbial MiniBio bundle is configured as follows

  • pH control is achieved with variable speed peristaltic pump, which can be configured for base or acid addition
  • Dissolved oxygen (DO) is controlled with air regulated by a solenoid valve and flow is set with a needle valve. 
  • Temperature control is achieved using a heating blanket wrapped around the bioreactor and a cold water valve that is connected to a heat exchanger in the bioreactor.
  • Foaming is reduced with a variable peristaltic speed pump with antifoam addition. 
  • Mixing is provided by a stirrer with 2 rushton, vortex impellers.

The cell MiniBio bundle is configured as follows

  • pH control is achieved with a fixed speed peristaltic pumps, which is configured as base and a CO2 solenoid valve for acid addition. CO2 Flow is set by a needle valve.
  • Dissolved oxygen (DO) is controlled with air regulated by a solenoid valve and flow is set with a needle valve. 
  • Temperature control is achieved using a heating blanket wrapped around the bioreactor.
  • Mixing is provided by a stirrer with marine impeller


MiniBio 250

MiniBio 500

MiniBio 1000

Total volume (ml)




Working volume (ml)




Minimum working volume (ml)




Aspect ratio total volume




Aspect ratio working volume




Dimensions (dxh, mm)

 180 x 400

 195 x 400

 200 x 550

Dimensions for autoclaving (dxh, mm)

 180 x 250

195 x 250 

 200 x 400

Drive system

Direct drive, lipsealed

Direct drive, lipsealed

Direct drive, lipsealed

Maximum stirrerspeed (rpm)

50 – 2000

50 - 1750

50 - 1500

Maximum impeller tipspeed (m/s)





Choice of Rushton and marine

Gas sparger

Porous sparger or L-type sparger

Gas overlay


Exhaust gas

Electrically cooled exhaust gas condenser
(evaporation <5% per day at 37°C @ 2vvm)


Fixed sample pipe with optional sampling system


Height adjustable drain pipe


4 fixed inlet ports and optional micro liquid injectors


Measurement: 8 mm classic pH sensor
Control: via acid pump (variable speed pump)or CO2 gas in combination with alkali pump (variable speed pump)


Measurement: 8 mm classic polarographic DO2 sensor
Control: via a combination of N2, Air, O2  (optional MFC) and agitation or nutrient addition (variable speed pump)


Measurement: Pt-100 sensor in thermowell in topplate
Control: electrical cooling and heating jacket via bioreactor wall


Measurement: Height adjustable conductivity based foam sensor
Control: Anti foam addition (variable speed pump)


Measurement: Height adjustable conductivity based level sensor
Control: variable speed pump for liquid addition or removal

Optional inlets

Septum, chemostat tube, liquid entry system


Key features

  • Intelligent control: The controller adaptively controls all key process parameters using an autotuning feature that automatically sets optimal PID control settings.  This feature ensures that optimal control performance is achieved quickly and without the need for advanced engineering expertise.
  • Access security: Password controlled access is provided for three levels of operator: operator, engineer and service engineer.  Each level has access to defined areas of functionality.
  • Software update: Updating software to the latest version is extremely simple and is achieved by connecting a USB memory stick to the controller.  The system automatically loads the update.
  • Advanced interface: Ethernet communication is used to connect the controller to a computer or network.  Communication to SCADA software (supervisory control and data acquisition) such as BioXpert is via Ethernet. The my-Control is OPC compliant which makes communication possible with any SCADA that meets this standard.
  • Remote communication: The biocontroller can be accessed remotely via the internet using standard Internet browsers to view and control the system.
  • Expandability: The my-Control can be configured to control virtually any bioprocess. Additional I/O (4x Analog In, 4x Analog Out, 8x Digital Out)or USB drivers (for scales or buglab) can be provided to communicate with external devices such as biomass monitors, optical density sensors, scales, gas analyzers, variable or fixed speed pump.
  • Documentation and validation: The my-Control has been developed and documented in compliance with regulations GAMP 5. This supports the full validation of the system for your process


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