Plaque Assay

A cell monolayer is treated with a dilution of a GFP-expressing virus. The cells are then coated with agar, which limits the virus spread to only neighboring cells.After incubation, GFP+ circles of infected cellsare counted for virus titration.

Related Instruments: Cytation 1, Cytation 5, Cytation 7, Lionheart FX, Lionheart LX, 405 TS, 405 LS

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Automating the Wash Steps of ELISpot Assays using BioTek’s 405 TS Microplate Washer

Enzyme-linked immunospot (ELISpot) assays are a flexible and powerful tool for investigating secreted molecules from whole cells, particularly for analyzing the immunological functions and responses of peripheral and lymphoid white blood cells. Here we describe a convenient and effective method for automating the most tedious aspects of conducting ELISpot assays using the versatile 405™ TS Microplate Washer.

Instrument: 405 TS, 405 LS
 

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Quantitation of monoclonal antibody infliximab in human plasma by LC-MS/MS using Fab-selective limited proteolysis nSMOL technology

Infliximab, known commercially as Remicade, is a chimeric IgG1 kappa monoclonal antibody (mAb) that targets tumor necrosis factor-alpha (TNF).
In studies conducted, a significant number of patients (30-40%) who did not respond to anti-TNF treatment were identified.

Instrument: LCMS-8060 Liquid Chromatography Mass Spectrometer (LCMSMS)

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Povidone, Formic Acid Test (USP/EP/JP)

Povidone (polyvinylpyrrolidone, PVP) is used in the pharmaceutical industry as a synthetic polymer vehicle for dispersing and suspending drugs. It additionally acts as a disintegrant and tablet binder.

The revision to the harmonized standard for Povidone has been approved and Formic Acid in Povidone is now required to be measured.

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Incorporation of a Novel, Automated Scratch Tool and Kinetic Label-Free Imaging to Perform Wound Healing Assays

The wound healing or "scratch" assay is one of the most highly used in vitro methods to monitor and quantify collective cell migration.

Instrument: Scratch Assay Starter Kit

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Pharmaceuticals and Human Science - Shimadzu Journal Cilt 2, Sayı 1

Pharmaceuticals and Human Science: This issue focuses on Pharmaceuticals and Human Science, and contains such articles as follows:

• An interview with Professor van Breemen, who is studying medicinal properties of natural products at the University of Illinois, College of Pharmacy, in Chicago, using advanced mass spectrometry

• Professor van Breemen's latest work of the effect of human exposure to bisphenol A in the environment

• Two interviews with Japanese pharmaceutical companies about Shimadzu's new technologies i-PDeA and i-DReC and technical reports of each technology

• In addition, it contains information on other applicable topics, as well as the latest news and applications.

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ProteaseTag ™ Active NE Immunoassay: A Rapid Test for the Quantification of Neutrophil Elastas Levels in Patients

Oliver Carney, BMG LABTECH Ltd, Aylesbury, UK; Kelly Moffitt and Charlene Robb, ProAxsis, Belfast, Northern Ireland, 02/2016

The neutrophil elastase is a biomarker of respiratory diseases such as cystic fibrosis (CF) or chronic obstructive pulmonary disease (COPD). A simple, rapid and accurate test for neutrophil elastase levels in patient samples would be a significant medical benefit. Established methods do not specifically verify the presence of the enzyme leading to a high value of false positives. In this application note we show how to use the ProteaseTag™ Active Neutrophil Elastase Immunoassay from the company ProAxsis which is based in Northern Ireland. 

The assay uses tags to specifically bind the enzyme of interest. Chromogenic detection is performed with the CLARIOstar® microplate reader which analysis the results and return qualified data automatically. 

Protease levels can be determined in sputum sol, bronchoalveolar lavage, wound exudate or serum free cell culture.

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Pharmaceutical Analysis and more - Shimadzu Journal Vol. 3

• Insights from our customer Professor Alexander Arnold from the Milwaukee Institute for Drug Discovery at the University of Wisconsin-Milwaukee
• The collaboration with Mitsubishi Tanabe Pharma Corporation, Japan for development of pharmaceutical products using Imaging Mass Spectrometry: iMScope TRIO
• Metabolic Studies of Drug Candidates for Neurological Disorders and Asthma Based on GABAA Receptor Subtype Selective Ligands using Mass Spectrometry
• Utility of High-resolution MALDI imaging in Drug Discovery: Histological Distribution of Gentamicin in Proximal Renal Tubules of Rats
• And many more ...

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Analysis of DNA Damage Size with LCMSMS

The aim of this study was to investigate the relationship between significant antioxidative metabolism in mice and to demonstrate the extent of oxidative damage over 8-hydroxy-2-deoxyguanosine (8-OHdG).

Instrument: LCMS-8040 Liquid Chromatography Mass Spectrometer (LCMSMS)

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Determination of Genotoxic Impurities in Drug by 2D-LCMS

Determination of genotoxic impurities in 2D-LCMSMS (Co-Sense) system.

Instrument:  LCMS-8040 Liquid Chromatography Mass Spectrometer (LCMSMS)

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Determination of Adhesion of Brass Starch to Aluminum Panels

Determination of adhesion of brass starch with centrifugal force.

Instrument: Hitachi Koki CS-FNX series / S110AT

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Cleaning Validation in Pharmaceutical Industry with TOC

In this study, a cleaning validation application for the pharmaceutical industry with Wet Chemical TOC Analyzer is included.

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