Shimadzu Corporation

MultiNA Microchip Electrophoresis System for DNA/RNA Analysis

  • Sample racks are compatible with 96-well PCR plate† and 12/8-strip PCR tubes.
  • Quartz Microchip, 23 mm separation channel length, on-chip electrodes (insert up to four microchips).
  • Electrophoresis Voltage Max. rated voltage: 1.5 kV, max. current: 250 µA
  • Analysis Cycle time approx. 80 s (using four chips) DNA standard analysis (DNA-1000/premixed).
  • LED-excited fluorescence detector (470 nm excitation wavelength)
  • Separation Size Range; 25 to 500 bp (DNA-500 Kit), 100 to 1000 bp (DNA-1000 Kit), 100 to 2500 bp (DNA-2500 Kit), 100 to 12000 bp (DNA-12000 Kit), Up to 28S rRNA (5.0 knt) (RNA Kit).
  • Resolution; 5 bp (25 to100 bp), 5% (100 to 500 bp), 10% (500 to 1000 bp), 20% (1000 to 12000 bp).
  • Sizing Accuracy;  ±5 bp (25 to 100 bp), ±5% (100 to 500 bp), ±15% (DNA-1000, DNA-2500, DNA-12000)
  • Min. Detection Limit; DNA analysis: 0.5 to 50 ng/µL (at 10mM Tris-HCI, containing 50 mM KCl and 1.5 mM MgCl2), RNA analysis: 25 to 500 ng/µL (total RNA), 25 to 250 ng/µL (mRNA) (10 mM Tris-HCI buffer, containing 1 mM EDTA)
  • Quantitation Range; DNA analysis: 0.2 ng/µL (at 10mM Tris-HCI buffer, containing 50 mM KCl and 1.5 mM MgCl2), RNA analysis: 5 ng/µL (total RNA), 25 ng/µL (mRNA) (at 10 mM Tris-HCI buffer, containing 1mM EDTA)
  • Quantitation Accuracy; DNA analysis: ±30% (at 10 mM Tris-HCI buffer, containing 50 mM KCL)
  • Quantitation Repeatability RNA analysis: CV 10% or less (CV 20% or less for eukaryotic-origin total RNA at concentrations of 150 ng/µL or more)
  • Data Processing; Batch display/detailed display of gel images/pherograms, automatic quantitation and size prediction by size markers, data searching, data import/export, manual editing and re-analysis

  • Sample racks are compatible with 96-well PCR plate† and 12/8-strip PCR tubes.
  • Quartz Microchip, 23 mm separation channel length, on-chip electrodes (insert up to four microchips).
  • Electrophoresis Voltage Max. rated voltage: 1.5 kV, max. current: 250 µA
  • Analysis Cycle time approx. 80 s (using four chips) DNA standard analysis (DNA-1000/premixed).
  • LED-excited fluorescence detector (470 nm excitation wavelength)
  • Separation Size Range; 25 to 500 bp (DNA-500 Kit), 100 to 1000 bp (DNA-1000 Kit), 100 to 2500 bp (DNA-2500 Kit), 100 to 12000 bp (DNA-12000 Kit), Up to 28S rRNA (5.0 knt) (RNA Kit).
  • Resolution; 5 bp (25 to100 bp), 5% (100 to 500 bp), 10% (500 to 1000 bp), 20% (1000 to 12000 bp).
  • Sizing Accuracy;  ±5 bp (25 to 100 bp), ±5% (100 to 500 bp), ±15% (DNA-1000, DNA-2500, DNA-12000)
  • Min. Detection Limit; DNA analysis: 0.5 to 50 ng/µL (at 10mM Tris-HCI, containing 50 mM KCl and 1.5 mM MgCl2), RNA analysis: 25 to 500 ng/µL (total RNA), 25 to 250 ng/µL (mRNA) (10 mM Tris-HCI buffer, containing 1 mM EDTA)
  • Quantitation Range; DNA analysis: 0.2 ng/µL (at 10mM Tris-HCI buffer, containing 50 mM KCl and 1.5 mM MgCl2), RNA analysis: 5 ng/µL (total RNA), 25 ng/µL (mRNA) (at 10 mM Tris-HCI buffer, containing 1mM EDTA)
  • Quantitation Accuracy; DNA analysis: ±30% (at 10 mM Tris-HCI buffer, containing 50 mM KCL)
  • Quantitation Repeatability RNA analysis: CV 10% or less (CV 20% or less for eukaryotic-origin total RNA at concentrations of 150 ng/µL or more)
  • Data Processing; Batch display/detailed display of gel images/pherograms, automatic quantitation and size prediction by size markers, data searching, data import/export, manual editing and re-analysis

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