Protein Sequencer - PPSQ

Benefits of using Edman Degradation for amino acid sequencing

• Guaranteed N-terminal sequence of proteins
• No sample pre-treatment required
• Differentiation of isobaric amino acids (leucine and isoleucine)
• Identification possible even from unknown proteins not registered in databases

Protein Sequencing Analysis

Complience with FDA 21 CFR Part 11

Simple, Easy-to-Use Data Analysis Functions

• Reprocessing of chromatograms required for data analysis
• Overlay of multiple chromatograms
• Automatic estimation of amino acid sequences

 

Protein Sequencing - Structure

Using Edman degradation, it derivatizes the amino acids of a protein in order from the N-termini, cleaves the derivatives, and from the HPLC retention times for these cleaved derivatives, identifies the amino acids.













 

Isocratic System in Protein Sequencing

Baseline Stability

PPSQ series protein sequencers separate PTH-amino acids isocratically. This improves baseline stability and allows high-sensitivity analysis of PTH-amino acids.

Retention Time Reproducibility

Isocratic sequence analysis provides more stable retention times. Therefore, peaks detected in previous cycles can be cancelled using substation chromatogram processing, making it easier to identify sequences.









 

Gradient System in Protein Sequencing

High-Sensitivity Analysis

A high-sensitivity flow cell enables high-sensitivity detection of PTH-amino acids, which allows for sequential analysis using trace samples.

        

Analysis Stability

Protein Sequencer has high analysis stability because it is equipped with a high-performance detector and a solvent delivery pump that provides excellent pumping performance, even in the micro flowrate range, combine to ensure high analysis stability. Because chromatograms with good reproducibility can be obtained, peaks detected in the previous cycle can be canceled by performing differential chromatogram processing. This enables easy identification of PTH-amino acids even from trace samples.