Shimadzu Corporation

PPSQ -51A/53A Automated Protein/Peptide Sequencing System

A fully PC controlled protein Sequencer, PPSQ- 51A/53A systems are equipped with especially designed, novel and precise reagent delivery system for the re-productive gas-phase Edman degradation cycles. The PPSQ-51A/53A consists of an Edman reaction unit, an on line PTH-amino acid Analyzer with LC pump and UV detector, and windows base software. New software improves ease-of-operation and data analysis features. Reduced noise level allows high-sensitivity detection of PTH-amino acids (noise level: 0.5 10-5 AU). Baseline stability of PTH-amino acid analysis in isocratic mode allows easy identification of sequences when analyzing microscale samples.
The PPSQ-51A is a single reactor system for high cost performance, while the PPSQ-53A is a triple reactor system for 3-sample continuous operation. Both system allow easy operation, easy interpretation, as well as high cost performance with high precision.

A fully PC controlled protein Sequencer, PPSQ- 51A/53A systems are equipped with especially designed, novel and precise reagent delivery system for the re-productive gas-phase Edman degradation cycles. The PPSQ-51A/53A consists of an Edman reaction unit, an on line PTH-amino acid Analyzer with LC pump and UV detector, and windows base software. New software improves ease-of-operation and data analysis features.

  • Edman degradation reaction method
  • PPSQ-51A: 46.5 min/cycle, PPSQ-53A: 48 min/cycle Reaction times varies by model.
  • PPSQ-51A Single Reactor model, PPSQ-53A Triple Reactor model (Supports consecutive analysis of 3 samples)
  • Glass fiber disk (8 mm dia.) or PVDF membrane sample immobilization method.
  • Reactor temperature control range is 10 °C above room temperature to 60 °C
  • Converter temperature control range is 10 °C above room temperature to 70 °C
  • Reagent/solvent delivery is carried by Nitrogen gas pressure
  • 7 Reagents/solvents

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