High Capacity RNA-to-cDNA™

• Super-efficient–up to 100x higher cDNA yield
• Super-sensitive–C_t values reduced by as many as 8 cycles for RT-qPCR
• Super-robust–transcribes even from degraded or inhibitor-containing RNA samples
• Super-fast–10 min reaction time

Optimum Performance for Real Time PCR

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription (RT) kit designed for optimum performance in real-time PCR with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and others. The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template quantities. The High Capacity RNA-to-cDNA Kit formulation reduces RT time by at least half, requires few pipetting steps, and delivering high cDNA yields.

The High Capacity RNA-to-cDNA Kit consolidates the components of the RT reaction into two tubes; 2X RT buffer and 20X enzyme mix, including RNase inhibitor. These components have been optimized to provide superior cDNA archiving for real-time gene expression analysis, and a more streamlined workflow is achieved by minimizing the the number of reagent additions.

Streamline your cDNA Synthesis while Maintaining Detection Sensitivity

The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template amounts. Amplification of a dilution series of the RNA concentration reference standard demonstrates exceptional reverse transcription and PCR efficiency across 11 orders of magnitude. This wide dynamic range allows one set of reaction conditions to detect transcripts from highly active as well as weakly expressed genes. The two-tube formulation reduces reaction time and requires fewer pipetting steps than with the High Capacity cDNA Reverse Transcription Kit, while maintaining high performance.

High Capacity cDNA Reverse Transcription Kit

The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single-stranded cDNA in a single 20 µL reaction. The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.













 

with RNase Inhibitor
High Capacity cDNA Reverse Transcription Kit

The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single-stranded cDNA in a single 20 µL reaction. This particular kit also includes 5 tubes of RNase inhibitor (200 µL each).

The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.












 

Features of this kit include:

• Linear target amplification for real-time PCR
• Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
• 10-fold greater dynamic range than other kits

Extensively tested with a variety of templates.

Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.

For more information please click.