ProFlex™ PCR Thermal Cycler Systems
Thermal Cycler with Ultimate Flexibility and Efficiency
With 5 interchangeable block formats, the ProFlex™ PCR Thermal Cycler fits the way you work today and tomorrow - maximize your throughput or run three experiments at once.
Easily Change Blocks to Meet Your PCR Needs
The ProFlex™ PCR Thermal Cycler has 5 different blocks, including a first-of-its-kind 3 x 32-well block, that can be changed with the flip of a switch. This block allows up to three experiments to be run simultaneously, completely independent of each other and without interference.
Dual 96-well (dual 96-well) and double 384-well (dual 384-well) blocks are available for your high-throughput sequencing needs, and a tool to support Applied Biosystems™ OpenArray plate technology for genotyping analysis on the Applied Biosystems™ QuantStudio 12K Flex Real-Time PCR System. Double flat block is available.
Precise PCR Optimization with VeriFlex Technology
VeriFlex Blocks provides a better-than-gradient approach to PCR optimization. With three separate Peltier blocks, you have precise control over the three temperature zones.
- Enables extremely precise determination of the optimum annealing temperature
- Helps eliminate the assumptions often associated with standard gradient cyclers
- Saves time by running up to three reactions with different annealing temperatures in the same PCR run
- Use your thermal cycler as an incubator; benefit from precise temperature control
What is VeriFlex™ Blocks temperature control?
Thermal cyclers using VeriFlex technology are constructed with three or more separate heating/cooling elements under each of the three or more segmented metal blocks, as shown in the figure. Each pair of heating/cooling elements and segmented metal blocks is completely isolated from the others. to help prevent heat interactions. As a result, VeriFlex technology provides a true linear temperature gradient across metal blocks with the ability to adjust up to six different temperatures, as shown in the Figure. The user can set each temperature zone independently, providing better control of temperature optimization.
ProFlex™ Secure Remote Access to Your Thermal Cycler
The ProFlex™ thermal cycler is a cloud-enabled tool that allows you to secure private access with a Thermo Fisher Connect account. Anywhere, anytime, from any mobile device or desktop computer, you can:
- Design and share protocols
- Program an instrument
- Start or stop a run
- Check the operating status
- It's easy to set up and comes with 10GB of free storage on your account.
Take Control With PCR Thermal Cycler Fleet Control Software
Securely manage multiple users, methods and tools with a single intuitive interface behind your firewall.
Technical Features
The ProFlex™ 96-Well and 2x96-Well PCR System allows you to:
- If your throughput needs to change, replace the 96-well 0.2 mL (and 2x96-well) block with another block type
- Access the system remotely (and conveniently) via a mobile app
- Program the device in seconds with the easy-to-use touchscreen interface
- Simulate your old device with Thermal Simulation modes
- Optimize your PCR quickly and efficiently with better-than-gradient VeriFlex™ blocks
Interchangeable Blocks
If your research needs change in the future, or if you share the system with someone who requires a different format, simply remove the 96-well block at the top of the instrument and replace it with another block type. This step typically takes less than 15 seconds and requires no tools or kits.
Mobile Application Link
Have you set up all your PCR reactions only to find out that the thermal cycler is already in use? Check availability with a mobile app before going to the lab. Also download experimental report files, get alerts and more! Download our PCR Essentials app updated with this functionality.
Simulation Modes
The ProFlex™ PCR system is equipped with Simulation Modes that emulate the ramp rate of your legacy thermal cycler. Simulation modes are available for MJ Research PTC 200, Bio-Rad® C1000, Bio-Rad® MyCycler™, Eppendorf® Mastercycler®, Applied Biosystems® 9700, Applied Biosystems® 9600 and Applied Biosystems® Veriti® systems.
VeriFlex™ Blocks: Enhanced PCR Functionality
VeriFlex™ blocks are controlled by multiple Peltier elements (6 individual Peltier elements per 96-well module). This gives you ultimate control over your block to run multiple reaction conditions simultaneously for precise, 'better-than-gradient' optimization experiments and precise incubations and other non-PCR workflows.
8.4' Touch Screen Control
The simple and intuitive touchscreen interface allows you to program with ease and limits the time you spend training yourself and others how to use the system.
| ProFlex™ 96-well Technical Specifications |
| Block Format |
Interchangeable |
| For Use With (Equipment) |
ProFlex™ PCR System |
| Peak Block Ramp Rate |
6.0°C⁄sec |
| Reaction Volume Range |
10-80 µl |
| Sample Ramp Rate |
± 4.4 °C⁄sec |
| Temperature Range (Metric) |
0 to 100.0 °C |
| Capacity |
1 x 96-well x 0.2 mL tubes |
| Display Type |
8.4 in. Color TFT LCD |
| Interface |
WiFi, Cloud, Ethernet |
| Power Requirements |
100-240V, 50-60 Hz Max: 950 VA |
| Block Configurations |
96-Well, 0.2 mL, 6-zone VeriFlex |
| Max. Ramp Rate |
6°C/Sec (Block), 4.4°C/Sec (Sample) |
| Thermal Uniformity |
<0.5°C (20 Sec After Reaching 95°C) |
| Thermal Accuracy |
±0.0.25°C (35°C to 99.9°C) |
| Dimensions |
33 cm (13 in) (W) x 27 cm (11 in) (H) x 57 cm (22 in) (D) |
| Reaction Speed |
Fast, Standard |
| Weight |
18.75 kg (41 lb) |
| Product Line |
ProFlex™ |
| ProFlex™ 2x96-well Technical Specifications |
| Block Format |
Interchangeable |
| For Use With (Equipment) |
ProFlex™ PCR System |
| Peak Block Ramp Rate |
3.0°C⁄sec |
| Reaction Volume Range |
10-100 µl |
| Sample Ramp Rate |
Standard mode: ± 1.6°C⁄sec |
| Temperature Range (Metric) |
0 to 100.0 °C |
| Capacity |
2 x 96-well x 0.2 mL tubes |
| Display Type |
8.4 in. Color TFT LCD |
| Interface |
WiFi, Cloud, Ethernet |
| Power Requirements |
100-240V, 50-60 Hz Max: 950 VA |
| Block Configurations |
96-Well, 0.2 mL, 6-zone VeriFlex |
| Max. Ramp Rate |
3°C/Sec (Block), 1.6°C/Sec (Sample) |
| Thermal Uniformity |
<0.5°C (20 Sec After Reaching 95°C) |
| Thermal Accuracy |
±0.0.25°C (35°C to 99.9°C) |
| Dimensions |
33 cm (13 in) (W) x 27 cm (11 in) (H) x 57 cm (22 in) (D) |
| Reaction Speed |
Fast, Standard |
| Weight |
18.75 kg (41 lb) |
| Product Line |
ProFlex™ |
| Format |
96-well plate, 12-strip wells, 0.2 ml tubes |
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