Features of C-tagXL Affinity Matrix include:
• Mild elution, making it suitable for pH-sensitive proteins
• Binding of the tagged proteins under denaturing conditions (like dissolved inclusion bodies)
• Excellent scalability
• Non-animal-derived

Main Characteristics

Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect C-tagXL affinity ligand
Ligand coupling method: epoxide coupling of the ligand
Binding capacity: 400 nmol/mL resin depending on flow rate, column height, and contact time
Elution conditions: Acidic: 20 mM citric acid or acetic acid, pH 3–4, or 0.1 M glycine pH 3.0; Neutral: 20 mM Tris, 2.0 M MgCl₂ pH 7; 20 mM Tris, 1.0 M NaCl, 50% (v/v) propylene glycol; 20 mM Tris 2 mM S-E-P-E-A.
Flow characteristics: 150–300 cm/h
Formulation buffer: 20%(v/v) ethanol



















CaptureSelect affinity resins for antibody purification
This image shows where various products bind the antibody or antibody fragment.

We have developed a unique portfolio of affinity resins that help you develop next-generation antibody therapeutics. These affinity resins can be used for clinical and commercial production and include:

• A platform solution for Fab production parts regardless of light chain type
• Scalable solutions for weak Protein A binding antibodies and antibody fragments
• Affinity resins offering gentle elution conditions for Fc-fusion proteins and pH-sensitive IgGs
• A cost-effective Protein A resin to help reduce clinical production cost


In addition to affinity resins, we also offer product-specific ELISAs for use in analytical assays to quantify any ligands potentially leached from the column and conjugated ligands.

CaptureSelect™ in Biosimilars and Recombinant Proteins

CaptureSelect™ helps you develop affinity resins, biosimilars, bio-enhanced products and other types of recombinant proteins.
These affinity resins can be used for clinical and commercial production. CaptureSelect protein purification products, non-antibody biomolecules
Exhibits affinity and specificity for target proteins for efficient one-step purification.

• Selectivity - high purity in one step; raw material independent
• Mild elution conditions - biological, retention of target activity
• Reducing process steps — helps reduce costs, allows higher throughput
• Effective removal of HCP, DNA, virus - high selectivity in a single capture step
• Protein Vaccine Development with C-tag and C tagXL Affinity Resin

One of the biggest bottlenecks in protein vaccine development is obtaining adequate amounts of high-quality and pure protein. The C-tag affinity tag allows for recombinant protein production by adding a small tag (4 amino acids) to the protein of interest.

The Thermo Scientific™ CaptureSelect™ C-tagXL Affinity Resin combines the unique selectivity of C-tag with the benefits of a robust and high-quality affinity matrix that provides high yield and purity in a single purification step.
 
C-tag advantages:

• Small inert tag, limiting effect on protein functionality
• Highly selective when coupled at the C-terminus of a protein
• Limits disadvantages of traditional labels such as lack of selectivity, heavy metal waste or limited reusability
• Provides high target yield and purity from complex mixtures (compared to His6 tag)
 
Advantages of the CaptureSelect™ C-tagXL affinity matrix:
 
• Provides high target purity and yield from complex mixtures
• Light elution preserving the protein of interest
• Scalable










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