Thermo Fisher Scientific

CaptureSelect™ FcXP Afinite Matriks

CaptureSelect FcXP Affinity Matrix is the next-generation CaptureSelect FcXL matrix, specifically designed for higher binding capacity.

The selectivity remains identical; the ligand recognizes the CH3 domain of all human IgG subclasses (IgG1, 2, 3, and 4), while maintaining the elution characteristics of CaptureSelect FcXL Affinity Matrix, but the binding capacity is increased (>40 g IgG/L).

CaptureSelect FcXP Affinity Matrix can be used for the purification of IgG and Fc fusion proteins directly from complex source materials and enables a robust and efficient purification process with excellent purity obtained in one step.

Features of this affinity matrix include:
• Mild elution, making it suitable for Fc fusion proteins
• Human-specific, no binding to bovine antibodies
• Excellent scalability
• Non-animal derived


Main Characteristics

Matrix: agarose-based, epoxide activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect FcXP affinity ligand
Ligand coupling method: epoxide coupling
Binding capacity: 40–50 g IgG/L resin depending on flow rate, column height, and contact time
Elution conditions: 20 mM citric acid or acetic acid, pH 3–4, or 100 mM Tris, 2.0 M MgCl2, 40% (v/v) propylene glycol, pH 7
Flow characteristics: 150–300 cm/h (up to 2 bar)
Formulation buffer: 20% (v/v) ethanol


It supports the development of next generation antibody therapeutics.

















 

Fab purification platform
Binding the CH1 field
Free light chain does not bind
Developed for Fab parts and bi-featured products
Binding CL - kappa area
High Dynamic Binding Capacity
Light elution
Developed for Fc fusion proteins and non-protein A binding IgGs
Binding the CH3 domain (Fc)
Light elution


Capture Select Antibody Selectivity - Next Generation FC Binder












 
Purification of recombinant human IgG, Fc fusion proteins and plasma-derived IgG
One-step purification from raw material with high purity and yield
Highly selective, binding occurs via the CH3 domain of human IgG
Recognizes all human IgG classes (IgG1-4)
High DBC: > 40 g/L (10% pass / 5 min residence time)
Light elution at pH 4.0 - 4.5 makes it suitable for Fc fusion proteins
Excellent scalability
Not of animal origin

 

CaptureSelect™ Technology: Antibody Selectivity and Affinity







 
 

Dynamic Binding Capacity at 10% Breakthrough















Capacities were measured with monoclonal Rituximab (titer: 5 g/l) and Polyclonal IgG (titer: 7 g/l) in 4 ml columns (0.5 x 20 cm).
Bound protein eluted using 20 mM Citric Acid pH 4.0
 

Elution Conditions









Elution efficiency with monoclonal antibody feed (Rituximab, titer 1.7 g/l) at 8 min residence time.
Bound mAb, 20 mM
Washed using Acetic Acid pH 4.0 and simplified with 100 mM Glycine pH 2.0.
(CaptureSelect FcXP affinity resin shows efficient elution at mild pH (>95% at pH4.0).)






























































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