CaptureSelect™ Human Albumin Affinity Matrix

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Thermo Fisher Scientific

CaptureSelect™ Human Albumin Affinity Matrix

The CaptureSelect™ Human Albumin Affinity Matrix is specifically designed for the purification of human albumin and albumin fusion proteins.

CaptureSelect™ provides a robust and efficient purification process with excellent purity achieved in one step, with high selectivity and yield using the Human Albumin Affinity Matrix.

The gentle elution conditions make the resin suitable for the purification of albumin fusion proteins, especially those that are pH sensitive.

Technical Specifications
Application Note

Features of AAV affinity matrix include:

• Highly selective for human albumin
• Unique elution characteristics allowing for gentle elution of albumin fusion proteins
• Excellent scalability
• Non-animal-derived

Main Characteristics

Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect™ HSA affinity ligand
Ligand coupling method: aldehyde coupling via NH₂ residues of the ligand
Binding capacity: ~15 mg albumin/mL resin
Elution conditions (neutral pH): 20 mM Tris, pH 7.4 with 2 M MgCl₂ or with 1 M NaCl + 50%(v/v) propylene glycol or with 1 M NaCl + 0.5 M L-arginine
Elution conditions (acidic pH): 0.1 M glycine, pH 3.0
Flow characteristics: 200–400 cm/h (up to 1 bar)
Formulation buffer: 20%(v/v) ethanol

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