CaptureSelect™ KappaXP Affinity Matrix

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Thermo Fisher Scientific

CaptureSelect™ KappaXP Affinity Matrix

CaptureSelect KappaXP Affinity Matrix is the improved version of CaptureSelect KappaXL Affinity Matrix, with a significant increase in binding capacity for both whole kappa monoclonal antibodies and kappa Fab's. It enables efficient elution, even at pH 6.0, and demonstrates an improved stability for caustic cleaning agents. It is specific for human antibodies or antibody fragments, without cross-specificity to bovine antibody.

CaptureSelect KappaXP matrix recognizes the kappa light chain of human antibodies, enabling purification of all kappa-containing Ig’s, Fab, and Fab2 fragments directly from complex sources and feed streams.

Technical Specifications
Application Note

CaptureSelect KappaXP Affinity Matrix features include:
• Purification of kappa monoclonal antibodies, Fab, and Fab2 fragments directly from complex source materials in a single step with high purity and yield
• Mild elution for Fab fragments and antibodies
• Human specific, no binding to bovine antibodies
• Excellent scalability
• Excellent pH stability up to 100 mM NaOH for limited exposure
• Non–animal-derived

Main Characteristics

Matrix: agarose-based
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect KappaXP affinity ligand
Ligand coupling method: epoxide immobilization of the ligand
Fab binding capacity: 20–30 g IgG Kappa-Fab/liter resin depending on flow rate, column height, and residence time
IgG binding capacity: 35–45 g IgG/liter resin depending on flow rate, column height, and residence time
Elution conditions: 20 mM citric acid or acetic acid, pH 3–4; 100 mM Tris, 1.5 M MgCl₂, pH 6
Flow characteristics: 150–300 cm/h (up to 2 bar)
Formulation buffer: 20% (v/v) ethanol

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