Western Blot Systems and Consumables

Thermo Fisher Scientific

SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate | Chemiluminescent Western Blot Detection

Thermo Scientific™ SuperSignal™  West Atto Ultimate Sensitivity Chemiluminescent Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for high attogram protein detection. SuperSignal West Atto substrate provides the highest-level sensitivity and better signal-to–noise ratios compared to other commercially available high-performance horseradish peroxidase (HRP) substrates.

SuperSignal™ West Atto

Ultimate Sensitivity Chemiluminescent Substrate Characteristics

  • Sensitivity: low-femtogram to high-attogram
  • Stability: 48 hours for working solution, one year for kit at 4°C
  • Compatibility: nitrocellulose and PVDF membranes
  • Signal duration: up to six hours
  • Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 µg/mL)
  • Recommended secondary antibody concentration: 1:100,000–1:250,000 dilution (4–10 ng/mL)
SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate is designed for superior sensitivity and signal-to–noise ratios for very low abundant targets or precious samples in western blot analysis. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Atto substrate enables detection of target proteins in amounts that are too small to be seen with typical high performance ECL substrates.

Chemiluminescence Detection

Chemiluminescent detection occurs when energy from a chemical reaction is released in the form of light. The two most common enzyme reporters that catalyze chemiluminescent reactions required for generating a recordable signal are horseradish peroxidase (HRP) and alkaline phosphatase (AP).











 
  SuperSignal West Pico Plus SuperSignal West Dura
  SuperSignal West Atto SuperSignal West Pico Plus SuperSignal West Dura
Choose when? Target is very low-abundant, sample is limited, or antibodies are limited Everyday applications; offers improved sensitivity over base level ECL Performing quantitative western blotting or maximum signal duration is needed
Features High Sensitivity Widest dynamic range Longest duration and linearity
Sensitivity Low femtogram to high attogram Low picogram to femtogram Mid femtogram
Duration 6 hours Up to 24 hours 24 hours
Recommend antibody dilutions
(1 mg/ml stock)		 1°: 1:5,000
2°: 1:100,000-1:250,000		 1°: 1:1,000
2°: 1:20,000-1:100,000		 1°: 1:5,000
2°: 1:50,000-1:250,000
Catalog No A38554 (20 mL)
A38555 (100 mL)
A38556 (200 mL)		 34579 (20 mL)
34577 (200 mL)
34580 (500 mL)
34578 (1 L)		 37071 (20 mL)
34075 (100 mL)
34076 (200 mL)



























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