Invitrogen™ iBlot™ 2 Gel Transfer Device
Standalone Unit for Faster, More Convenient Transfers
The iBlot™ 2 Dry Blotting System is designed for a high degree of convenience. The iBlot™ 2 Dry Blot Transfer Device is a true stand-alone, plug and play system with an integrated power supply, pre-packaged ready-to-use transfer stacks. Installation, disassembly and cleaning takes only a few minutes compared to the much more cumbersome and hands-on steps required for wet tank transfer. iBlot™ 2 Dry-Gel Transfer Blot System has a simple touch screen interface and pre-programmed optimized transfer programs with the option to customize the programs according to your needs.
High Transfer Efficiency
In addition to its ease of use and convenience, the iBlot™ 2 Dry Western Blotting System offers high-throughput protein transfers for high downstream detection sensitivity – higher than wet and semi-dry transfer methods in most cases. As a result, it helps you achieve more accurate detection using fewer samples. Transfer the proteins in 7 minutes and quickly move on to the rest of your western blotting.
Fits Your Gel Size and Works with Your Gel Chemistry
iBlot™ 2 Dry Blotting System with multiple polyacrylamide gel sizes, including midi size (8 x 13 cm), E-PAGE 48 and 96 gels (13.5 x 10.8 cm), and mini gels (8 x 8 cm) It is designed to work. The system is also compatible with all protein gel chemistries (tris-glycine, bis-tris, tris-acetate and tricine).
iBlot™ 2 Dry Transfer Device Features
- Complete protein transfer in seven minutes or less
- High detection sensitivity and even transfer
- Increased blotting reliability and reproducibility
- A simple, user-friendly system
- Pre-programed with six voltage methods that allows blotting using different combinations of volts and time
- Ability to create custom programs
- Built-in tutorials and application notes
- Transfer proteins from mini-, midi-, and E-PAGE-sized gels
How the iBlot™ 2 Dry Blotting System works?
Buffer ion reservoirs are incorporated into the gel matrix of transfer stacks instead of buffer tanks or soaked papers. The high density of ions in the gel matrix enables rapid protein transfer. During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen).
With the iBlot™ 2 Dry Blotting System, transfer time is reduced by the shortened distance between electrodes, high field strength, and high current. Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to our unique de-bubbling design that promotes even and complete transfer. With the iBlot™ 2 system, there is no need to prepare buffers, pre-treat your gel, or clean up after blotting. The total preparation and run time is normally less than ten minutes per blot.
Specifications
|
| Capacity |
1-2 mini, or 1 midi, or 1 E-PAGE gel |
| Compabilitiy |
1.0-1.5 mm thick gels |
| Transfer Time |
6-10 min. |
| Transfer Buffer |
No need |
| Programs |
Pre-programmed with six methods for transfer of proteins from various gel types or create custom programs |
| Power Supply |
Internal |
| Dimensions |
37x20x11 cm |
| Weight |
2.3 kg |
| Electrical Parameters |
100-240 V, 50/60 Hz, 6.3 A |
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