Gibco® CHO CD EfficientFeed™ A liquid nutrient supplement is an animal origin-free, chemically defined complete feed formulation that provides up to 2-5 fold improvement in titers used in fed batch versus batch culture. It contains no proteins, hydrolysates, or components of incompletely defined composition.

Gibco® EfficientFeed™ A+ (Plus) AGT™ nutritional supplement is a dry format one-piece supplement for fed batch addition to the culture of multiple cell lines to aid in process improvement and maximize bioreactor utilization.

The liquid format is designed for small scale use and experimentation. This product is also available in AGT™ format for large scale manufacturing and ease of use.

• Expect up to 2- to 5-fold increases in productivity
• Avoid issues associated with the use of hydrolysates and other alternatives while gaining superior performance
• Ready for larger scale use with convenient packaging options (10 L and 100 L)

Gibco® CHO CD EfficientFeed™ B liquid nutrient supplement is a product for fed batch addition to the culture of multiple cell lines. CHO CD EfficientFeed™ B liquid nutrient supplement is an animal origin-free, chemically defined formulation that contains no proteins, hydrolysates, or components of incompletely defined composition.

The liquid format is designed for small scale use and experimentation. This product is also available in AGT™ format for large scale manufacturing and ease of use.

• Expect up to 2- to 5-fold increases in productivity
• Avoid issues associated with the use of hydrolysates and other alternatives while gaining superior performance
• Ready for larger scale use with convenient packaging options (10 L and 100 L)

 

Gibco® EfficientFeed™ C+ (Plus) AGT™ Supplement is a dry-format single-part supplement for fed batch addition to the culture of multiple cell lines to assist with process development and maximization of bioreactor utilization.

This product is designed to work with CHO cell lines expressing endogenous glutamine synthetase (GS) as a single feed without the need for additional GS supplements. Compared to the original CD EfficientFeed™ C AGT™ nutrient supplement, EfficientFeed™ C+ supplement has components that are uniquely combined to allow simpler pH-neutral reconstitution, as well as the ability to super-concentrate the supplement from 81.2 g/L (1X) to 162.4 g/L (2X) for reduction of product dilution or increased titers with additional feeding, when appropriate.

The product is an animal origin-free, chemically defined formulation that contains no proteins, hydrolysates, or components of incompletely defined composition.





















 

Criteria for Supplementation

Specific CHO cell lines and clones react differently to alternative supplementation protocols. Metabolic needs vary and it is essential for optimal growth and/or productivity to determine optimal nutrient supplementation protocols for each cell line or clone. You may find that two or more different feeds are needed. 

Selecting the feed or combination that may fit best for your process

A chart is provided below to help in the selection of Gibco media and feeds to pair for testing based on cell type.
















 

Simplified Handling to Reduce Risk in Scale-up

The ability to scale up using AGT dry format provides easier handling and storage in manufacturing and other bioprocess settings. Fewer parts and reconstitution steps mean fewer opportunities for operator error.
Further, the ability to store this feed, even when super-concentrated, for the length of a bioreactor run at room temperature in the dark may lessen the chance of contamination by reducing the number of connections to the bioreactor to one or two .
Customization of other feeding formulations is possible should that option suit your needs better after trying this kit. You can contact your local Field Application Scientist through your Account Manager to learn more about our customization capabilities.

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