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Thermo Fisher Scientific

Invitrogen™ Novex™ Tricine Protein Gels - Protein Gel Electrophoresis / Western Blot

The Invitrogen™ Novex™ Tricine Protein Gel System is a modification of the traditional tris-glycine gel system that uses a discontinuous buffer system specifically designed for the solubilization of low molecular weight proteins.

In the Thermo Fisher Scientific™ Invitrogen™ Tricine Gel system, tricine replaces glycine in the working buffer, resulting in more efficient stacking and destacking for low molecular weight proteins and higher solubility of smaller peptides.

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Technical Specifications
Application Note

Invitrogen™ Novex™ Tricine Protein Gels

Features Novex Tricine Protein Gels

Increased resolution of proteins with molecular weights as low as 2 kDa
Improved compatibility with direct sequencing of proteins after transferring to PVDF
Minimized protein modificationdue to the lower pH of the tricine buffering system

Formulation

Invitrogen Tricine gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. Our Tricine gels have a 4% stacking gel and do not contain SDS. The Tricine system requires SDS in sample and running buffers for best results.
Novex™ 16%, Tricine, 1.0 mm, Mini Protein Gel, 15-well

Novex™ 16%, Tricine, 1.0 mm, Mini Protein Gel, 15-well

Choose the Right Tricine Gel for Protein Separation

Invitrogen Tricine gels come in three polyacrylamide concentrations of 10%, 16%, and a gradient of 10–20%. Select from our many well formats, including 10-, 12-, and 15-well. Tricine gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, we recommend Tricine SDS Sample Buffer and optimal separation use Tricine SDS Running Buffer.

For transfer of proteins to a membrane, we recommend using the Novex Tris-Glycine Transfer Buffer if performing a traditional wet transfer using the XCell II Blot Module or the Mini Blot Module. Rapid semi-dry transfer can be performed using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

How Tricine Gels works?

In the traditional tris-glycine protein gel system, the resolution of smaller proteins (<10 kDa) is hindered by the continuous accumulation of free dodecyl sulfate (DS) ions from the SDS sample and running buffers in the stacking gel, which causes mixing of the DS ions with smaller proteins and results in fuzzy bands and decreased resolution. The mixing also interferes with the fixing and staining of smaller proteins.
The Novex Tricine Gel System uses a low pH in the gel buffer and substitutes tricine for glycine in the running buffer. The smaller proteins and peptides that migrate with the stacked DS ions in the tris-glycine gel system are well separated from DS ions in the Novex Tricine Gel System, offering sharper bands and higher resolution.

Tricine Protein Gels Specifications
Available Gel Sizes	Mini: 8 cm x 8 cm (1.0 mm thick)
Storage Conditions	2–8°C
Shelf Life	1-2 month
Recommended Sample Buffers	Tricine SDS sample buffer
Recommended Running Buffers	Tricine SDS running buffer
Recommended Transfer Buffers	Tris-glycine transfer buffer
Gel Chemistry	Tricine
Available Polyacrylamide Concentrations	10%, 16%, 10-20%
Separation Range	2–20 kDa
For Use with (Equipment) Mini Gels	Mini Jel Tank or XCell SureLock Mini-Cell
Mode of Separation	Molecular Weight

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