SuperScript IV Rever Transcriptase is a proprietary MMLV RT mutant with reduced RNase H activity as well as increased operability, thermostability, and inhibitor resistance for precise and efficient full-length cDNA synthesis. Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with reliable Platinum Hot-Start technology for highest success in PCR.










The combination of these two engineered enzymes provides enhanced RT-PCR yields and fidelity as well as amplification of longer templates compared to other one-step Real Time PCR (RT-PCR) kits. The inclusion of the ezDNase enzyme for use upstream of the RT step further accelerates the RT-PCR workflow through a highly simplified genomic DNA extraction step. (This one-step RT-PCR system is also available without ezDNase.)

Advantages of SuperScript™ IV One-Step RT-PCR System

High Specificity and Yield with Shortest Protocol

With the innovative two-phase, hot-start activation mechanism, the SuperScript IV One-Step RT-PCR System generates specific product at high yields in half the reaction time compared to other commercial kits.
 

Amplification of long targets with high specificity in dramatically shorter time. Detection of 7.8 kb target from total HeLa RNA using SuperScript IV One-Step RT-PCR System, Invitrogen SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity, and supplier QI, N, QU, T, R, and BL one-step RT-PCR products. Reactions were performed according to supplier’s recommendations. Total one-step RT-PCR reaction times are indicated in hours:minutes. RNA target failed to amplify with products from suppliers QI and BL.

Superior Sensitivity - down to 0.01 pg RNA

The high sensitivity of SuperScript IV One-Step RT-PCR System enables detection of low-abundance RNA targets. High sensitivity is advantageous in experiments where there is a limited amount of starting material or the concentration of target RNA in the sample is low.

High sensitivity and reliable target detection from low amounts of input RNA. Amplification of 0.35 kb fragment from 0.01 pg, 1 pg, 10 pg, 10 ng, and 100 ng total HeLa RNA with the SuperScript IV One-Step RT-PCR System, SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity, and supplier QI, N, QU, T, BL, and R one-step RT-PCR products.

 

 

Broad Range of RNA Target Lengths - up to 13,8 kb

Due to the high processivity of SuperScript IV RT and Platinum SuperFi DNA Polymerase, the SuperScript IV One-Step RT-PCR System enables detection of a broad range of target lengths.
 

Versatility across a broad range of target lengths. Detection of human RNA fragments ranging from 0.2 to 13.8 kb with the SuperScript IV One-Step RT-PCR System.

 

 

Resistance to Inhibitors 

The SuperScript IV One-Step RT-PCR System is able to withstand the effect of common RT and PCR inhibitors such as copurified compounds from biological samples or reagents used for RNA purification. This exceptionally robust system minimizes dependency on RNA-sample purity for reliable results.

Resistance to inhibitors. Detection of a 1 kb RNA target from total HeLa RNA using the SuperScript IV One-Step RT-PCR System, SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity, and supplier QI, N, QU, T, BL, and R one-step RT-PCR products in reaction mixtures containing: 1–No inhibitor 2–Heparin (0.18 µg/uL) 3–Xylan (2.5 µg/uL) 4–Humic acid (0.02 µg/uL 5–LiCl (2 µg/uL). All kits except the SuperScript IV One-Step RT-PCR System were inhibited with indicated amounts of inhibitors.

 

Integrated gDNA Removal 

• RNA purification methods, including protocols with DNase digestion on-column, often fail to remove gDNA completely. Amplification of contaminating gDNA can cause non-specific or misleading one-step RT-PCR results.
• Traditional gDNA decontamination protocols with DNase I include time consuming DNase inactivation or removal steps under conditions that can damage RNA and affect results.
• The new SuperScript IV One-Step RT-PCR System with ezDNase Enzyme allows efficient, fast, and gentle (5 min at 37°C) gDNA elimination from RNA samples and ensures the highest accuracy and confidence in one-step RT-PCR results.

 

Multiplex One-Step Real Time PCR

With its high robustness SuperScript IV One-Step RT-PCR system can amplify several targets  in the same multiplex one-step RT-PCR reaction with high efficiency and specificity. 

Simultaneous amplification of up to 4 targets using SuperScript IV One-Step RT-PCR System. One or more targets of increasing sizes (528, 997, 3,009, and 4,497) was amplified using total HeLa RNA as template.

Invitrogen™ SuperScript™ IV One-Step RT-PCR and One-Step Real Time PCR Systems with ezDNase combine the high processing capability of SuperScript IV Reverse Transcriptase (RT) with the high accuracy of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance. With this convenient one-step formulation and gene-specific primers, you can perform both cDNA synthesis from total RNA or mRNA and PCR amplification in a single tube.

For more information please click.