• Differentiating selectivity and superior resolution capability which helps improve yield and purity, reduce cost of goods (COG)
• High capacity for a range of molecules leading to reduced column size and smaller footprint
• Use of lower salt concentrations and weaker lyotropic salts allowing for increased process optimization flexibility with lower conductivity while maintaining superior resolution
• Flow rate independent performance
• Robust stability

Key Features

• 3 unique resins covering a wide range of hydrophobicity
• High resolution even under lower conductivity conditions
• High dynamic binding capacity and superior pressure flow characteristics

Separation comparison of POROS benzyl resin at different flow rates shows good solubility and flow rate independent performance.

Experimental details: Load buffer: 1.7 M ammonium in 50 mM sodium phosphate sulfate, pH 7; buffer gradient: load buffer to 50 mM sodium phosphate at 10 CV, pH 7; format: 0.46 cm D x 20 cm L; flow rate: 200, 400, 600 cm/h.
Protein mix: Ribonuclease A, lysozyme and chymotrypsinogen A.













High performance and solubility of POROS™ Benzyl Ultra resin with low conductivity buffer. POROS™ Benzyl and Benzyl Ultra resins are designed for use in low salt applications without sacrificing performance.
Protein mix: Ribonuclease A, lysozyme, chymotrypsin and chymotrypsinogen A.

Key Applications

Monoclonal antibodies, bispecific antibodies and antibody-drug conjugates (ADCs)
Removal of product-related impurities and aggregate
Plasmids, RNAi and oligonucleotides

** Separation of a standard protein mixture using POROS Ethyl, POROS Benzyl and POROS Benzyl Ultra resins.
Column size: 0.46cmD X 20cmL; Protein Mixture: Ribonuclease A, Lysozyme, Chymotrypsin and Chymotrypsinogen in 1.7M ammonium sulfate, 50mM sodium phosphate pH 7.0; Gradient Elution: 1.7M ammonium sulfate / 50 mM sodium phosphate pH 7.0 to 50 mM sodium phosphate pH 7.0, 10 column volumes (CV); Flow rate: 100 cm/h.

The new 50 µm resin backbone consists of cross-linked poly (styrene-divinylbenzene) with a unique pore structure that enables rapid bulk transport and increased productivity.
The particle surface is then coated with a novel polymer coating derived from a series of hydrophobic ligands for flexible purification process design.
POROS™ HIC resins provide a major improvement in the purification toolbox for superior performance and contamination removal regardless of flow rate.

• It provides different selectivity and superior solubility ability, which helps to increase yield and purity and reduce product cost.
• High capacity for a range of molecules leads to smaller column size and smaller footprint.
• The use of lower salt concentrations and weaker lyotropic salts provides increased process optimization flexibility with lower conductivity while maintaining superior solubility
• Flow rate independent performance
• Robust stability

POROS™ Hydrophobic Interaction Resin Types

POROS™ Ethyl Hydrophobic Interaction Chromatography Resin









POROS™ Benzyl Hydrophobic Interaction Chromatography Resin











POROS™ Benzyl Ultra Hydrophobic Interaction Chromatography Resin



 

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