Bis-Tris chemistry in NuPAGE Bis-Tris and Bolt Bis-Tris Plus Gels also promotes stability of the polyacrylamide gel matrix, enabling room temperature storage, which helps save on the need for refrigerator space.

Invitrogen Bis-Tris Protein Gels

Sharp, Straight Bands

Neutral-pH buffers in NuPAGE Bis-Tris and Bolt Bis-Tris Plus Gels deliver sharp straight bands. During separation, the gels operate close to pH 7. In the Laemmli system (Tris-glycine), the gel is run at basic pH (pH ~9.5). At high pH the residual unpolymerized acrylamide can react with cysteine and lysine residues of the proteins being separated. This may affect downstream analysis such as western blot transfer. At the neutral pH of NuPAGE Bis-Tris and Bolt Bis-Tris Gels, these reactions take place several orders of magnitude more slowly than at the basic pH of Tris-glycine gels, resulting in sharper bands and better resolution.

Better Sample Integrity

Sample preparation is a crucial step in successful protein analysis. The pH of the traditional Laemmli-style sample buffer changes from 6.8 to 5.2 when heated at 100°C. This lower pH is known to induce aspartyl-prolyl (Asp-Pro) peptide bond cleavage, which leads to protein degradation. By maintaining a >7.0 pH environment, the Invitrogen NuPAGE LDS Sample Buffer and Bolt LDS Sample Buffer preserve protein integrity by minimizing this Asp-Pro cleavage.

As seen below, the sample integrity is maintained throughout electrophoresis with the Invitrogen NuPAGE system; protein degradation is seen in samples prepared with Laemmli (Tris-glycine) sample buffer. The Invitrogen NuPAGE Antioxidant running buffer additive greatly minimizes protein oxidation during electrophoresis and keeps reduced protein bands sharp and clear.
 

Bolt Bis-Tris Plus Gel

High Capacity WedgeWell Sample Wells

The unique wedge-shaped well in every Bolt gel provides higher loading capacity, so you can load up to twice as much protein sample in every well.

Detect proteins even in dilute samples or measure expression of low-abundance proteins. With more room to load your sample, you no longer have to worry about sample spillover and cross-well contamination on your western blots. Gel loading is also easier with no need to use special gel-loading pipette tips.

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