Thermo Fisher Scientific

SuperSignal™ West Dura Extended Duration Substrate (ECL) | Chemiluminescent Western Blot Detection

Thermo Scientific SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

SuperSignal™ West Dura

Features of Extended Duration Chemiluminescent Substrate

  • Sensitivity: mid-femtogram
  • Stability: 24-hr working solution stability; 1-year kit stability at room temperature
  • Compatibility: nitrocellulose and PVDF membranes
  • Signal duration: 24 hours
  • Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 µg/mL)
  • Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)

Chemiluminescence Detection

Chemiluminescent detection occurs when energy from a chemical reaction is released in the form of light. The two most common enzyme reporters that catalyze chemiluminescent reactions required for generating a recordable signal are horseradish peroxidase (HRP) and alkaline phosphatase (AP).
 


 

ECL Substrates for High Sensitivity Western Blot Detection

Enhanced chemiluminescence (ECL) substrates, are HRP substrates that are typically two-component systems consisting of a stable peroxide solution and an enhanced luminol solution. To make a working solution, the two components are mixed together. When incubated with a blot on which HRP-conjugated antibodies (or other probes) are bound, a chemical reaction emits light at 425 nm which can be captured with x-ray film, CCD camera imaging devices and phosphorimagers that detect chemiluminescence. Light emission occurs only during the enzyme-substrate reaction; therefore, once the substrate in proximity to the enzyme is exhausted, signal output ceases.

We offer six types of ECL substrates for western blot detection with horseradish peroxidase enzyme (HRP). Each containing different enhancers to increase signal, linearity and duration compared to other western blot detection systems. 

  SuperSignal West Pico Plus SuperSignal West Dura
  SuperSignal West Atto SuperSignal West Pico Plus SuperSignal West Dura
Choose when? Target is very low-abundant, sample is limited, or antibodies are limited Everyday applications; offers improved sensitivity over base level ECL Performing quantitative western blotting or maximum signal duration is needed
Features High Sensitivity Widest dynamic range Longest duration and linearity
Sensitivity Low femtogram to high attogram Low picogram to femtogram Mid femtogram
Duration 6 hours Up to 24 hours 24 hours
Recommend antibody dilutions
(1 mg/ml stock)
1°: 1:5,000
2°: 1:100,000-1:250,000
1°: 1:1,000
2°: 1:20,000-1:100,000
1°: 1:5,000
2°: 1:50,000-1:250,000
Catalog No A38554 (20 mL)
A38555 (100 mL)
A38556 (200 mL)
34579 (20 mL)
34577 (200 mL)
34580 (500 mL)
34578 (1 L)
37071 (20 mL)
34075 (100 mL)
34076 (200 mL)



























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