CellInsight™ CX5

Our High-Content Screening (HCS), also known as High-Content Analysis (HCA), or High-Content Imaging platforms are designed for exceptional single-cell analysis capabilities and lightning-fast time-to-data. Perform unbiased spontaneous phenotyping with intact, fixed, or live cells derived from monolayers to spheroids. 
 
Featured in over 2,000 peer-reviewed publications, the CellInsight platform and HCS Studio software combine multi-parameter fluorescence microscopy with mathematical algorithms that identify and measure each cell within the fields of view. Conducting imaging and analysis in parallel provides the fastest time-to-data possible, a key advantage for high-throughput screening applications. Recent advances in laser-based focusing, confocal spinning disc performance, camera sensitivity and algorithm processing have accelerated throughput so that an entire 96-well plate can be scanned and quantified in under four minutes.

Features of CellInsight™ CX5 HCS Platform

• Easy to Use—quickly build and optimize image-based assays with included Thermo Scientific™ HCS Studio™ Cell Analysis Software
• Flexible—designed to work with or without fluorescent labeling: The CellInsight CX5 HCS Platform can use transmitted light, as well as fluorescence, to allow users to focus on visible and/or fluorescent targets
• Affordable—automated high content imaging for all budgets
• High quality optics—Light sources, lenses, filters, and CCD detector from the top names in imaging. The five color light engine insures accuracy and stability of source lighting.
• Virtually maintenance–free operation due to state-of-the-art design and construction.
• Performance—scan and analyze a 96-well plate in under four minutes
• Compact—fits on a bench and is less than 2 ft. on all sides

 

BPAE cells imaged using the CellInsight CX5 platform BPAE cells imaged using the CellInsight CX5 platform and stained with DAPI (blue), Alexa Fluor 568 phalloidin (red), and goat anti–mouse IgG (H+L) secondary antibody, Alexa Fluor 488 conjugate (green).	BPAE cells imaged using the CellInsight CX5 platform BPAE cells imaged using the CellInsight CX5 platform and stained with DAPI (blue), Alexa Fluor 568 phalloidin (red), and goat anti–mouse IgG (H+L) secondary antibody, Alexa Fluor 488 conjugate (green).
Brightfield imaging of C. elegans with CellInsight CX5 instrument Mixed brightfield-widefield imaging of Caenorhabditis elegans in vivo samples using the CellInsight CX5 instrument.	Multicolor fluorescent image of HEK293 cells captured using CellInsight CX5 instrument  Widefield immunofluorescent imaging of HEK293 cells using the CellInsight CX5 instrument.

Capabilites

• Make intracellular to multicellular morphology measurements while measuring intensity of specific reporter molecules.
• Collect only the images and data you select and make multiple measurements per object, over time, if desired
• Measure subpopulations and reject undesired objects automatically
• Comprehensive pre-validated and user configurable image analysis without user scripting or programming
• Exploratory assay development tools and specific biology optimized tools built into the HCS Studio™ Cell Analysis Software
• Define biological thresholds for outputs based on control populations
• Ability to create multi-parameter gates from individual output measurements

 

Hardware and Software

• Stable, reliable light source reduces variability, increases data quality, and decreases operating costs
• Fluorescence options which include near-IR wavelength and compatibility with many common fluorochromes and fluorescent proteins
• Proprietary Thermo Scientific™ AccuFocus™ System is fast, yet flexible, for imaging cells, organelles, and low contrast targets
• Unique intelligent acQuisition (iQ) real time analysis feedback to optimize image collection and data quality
• HCS Studio Cell Analysis Software is an integrated instrument control, visualization and analysis software package for novice to advanced users
• Features Thermo Scientific™ Store Express Image and Database Software using a 10GB SQL™ database
• Software output features image/data portability so results can be imported into third-party software for documentation
• Compatible with SBS standard microplates (6-1536 well) and slides

Compatible with Thermo Scientific™ Orbitor RS Plate Mover and other Thermo Scientific™ Laboratory Automation Solutions
 

Specifications
Camera	Photometrics High-Resolution Fluorescent Camera: High-resolution, thermoelectrically cooled to 0°C FOV 2208 x 2208-pixel array 4.54-micron pixel size 75% peak Quantum Efficiency (QE)
Light source	LED, solid-state five-color light engine used with provided filter sets offers the following excitation/emission capabilities (Ex/Em, in nm): 386/440 (e.g., Alexa Fluor 350 dye) 485/521 (e.g., Alexa Fluor 488 dye) 560/607 (e.g., Alexa Fluor 594 dye) 650/694 (e.g., Alexa Fluor 647 dye) 740/810 (e.g., Alexa Fluor 750 dye)
Objectives	Standard (Olympus objectives): 10x (0.30NA) 20x (0.4NA) Optional (Olympus objectives): 2x (0.08NA) 4x (0.16NA) 40x (0.95NA) 60x (0.90NA)
Image compability	JPG, BMP, GIF, PNG, TIF, C01, DIB
Dimensions	Height: 37.2 cm (14.6 in) Width: 41.4 cm (16.3 in) Depth: 59.4 cm (23.4 in)
Weight	45 kg (99.2 lb)
Data management	Compatible with Thermo Scientific Store Image and Database Management Software
Software	Thermo Scientific HCS Studio 4.0 Cell Analysis Software
Wattage	300 W

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