Thermo Fisher Scientific

Thermo Scientific™ CellInsight™ CX7 LZR High Content Analysis - Screening (HCA-HCS) Platform

The Thermo Scientific™ CellInsight™ CX7 LZR platform seamlessly integrates advanced scanning technologies to deliver superior performance for lightning-fast discovery in the life sciences. Designed to meet the demanding needs of emerging life science models, the CX7 LZR incorporates both laser-based fluorescent illumination and integrated Nipkow spinning disk confocal technology for broadcast quality imaging of a wide variety of samples, from simple monolayers to thick spheroid samples.

Dual-mode software and laser-based autofocus methods add to the platform's diverse capabilities for probing simple to complex models. On-the-fly phenotyping provides parallel image capture and analysis for multiplexed cytometry measurements in real time. The Thermo Scientific Amira software option provides 3D analysis of the most complex research models, from 3D morphological tracing in neurons to the co-localizations of immune and cancer cells within tumors to enable the next generation of translational research.

CellInsight™ CX7 LZR 

In addition to the common features of all high-content screening platforms, the CellInsight CX7 LZR platform offers:
  • Laser-based illumination 

Seven independent lasers aligned with multiplexable fluorescent cellular labeling for the best performance in speed and image quality. Laser illumination provides flatter and brighter images with low background.
 

  • Autofocus
Dual Mode Autofocusing Software and Laser Autofocus modes accommodate the widest variety of sample types.
 
  • Extremely bright illumination
Penetrate thick samples during widefield or confocal 3D imaging.
 
  • Laser light engine

Illuminates the field evenly resulting in the best image quality appropriate for quantitation. Up to 6X increase in 3D confocal screening times compared to LED illumination.

  • Short exposure times and laser autofocus capabilities
Speeds up the acquisition of images. 
 
  • Near-IR (785 nm) laser excitation
Expand your multiplexing capabilities with near-IR (785 nm) laser excitation.
 

Sample Data

Multicolor immunofluorescent imaging with confocal option from CellInsight CX7 LZR instrument. Multicolor fluorescent image of spheroid imaged using CellInsight CX7 LZR and Invitrogen antibodies and dyes.
Immunofluorescence image of human neurons
Confocal image obtained with CellInsight CX7 LZR instrument. Neurons were fluorescently stained with both DAPI and MAP2 (560 nM) to counterstain the nucleus and cell body/neurites, respectively. (60X objective (0.9NA); Nipkow spinning disc at 40 micron pinhole setting).
Immunofluorescence image of HeLa spheroid
HeLa spheroids labeled with Ki67 antibody followed by Invitrogen Alexa Fluor 647 and Alexa Fluor 488 phalloidin and Hoechst 34580 stains for nuclei. Imaged on the CellInsight CX7 LZR instrument using a 10x objective and 70 micron pinhole setting.

In hydroxyurea treated spheroids, actively proliferating s-phase cells disappear (EdU negative)

Imaging proliferating cells in spheroids using CX7 LZR platform
Imaging and analyzing proliferating cells in spheroids using CX7 LZR HCS instrument.

Spheroid segmented as whole object and EdU positive cells segmented in the spheroid for quantitation




 

Imaging and detecting spheroid size by segmenting and quantitating EdU and CX7 LZR platform
Segmentation and quantitation of EdU and spheroid size using CellInsight CX7 LZR system.

Features of CellInsight CX7 HCA Platform

  • 7-channel fluorescence imaging
  • 5-channel bright field imaging
  • 7-channel confocal imaging
  • Software and laser-based autofocus for consistent scan times
  • Configured for fully automatic plate processing and scanning

Capabilities

  • Addresses hundreds of assay types with flexible software and hardware tools
  • Scan and analyze microscope slides or most SBS-standard format microplates
  • Use HCS Studio software for integrated instrument control and data analysis
  • Achieve the fastest time to results by using intelligent acQuisition (iQ) real-time analysis feedback
  • Compatible with Molecular Probes™ fluorescent detection reagents


Versatility and Accelerated Output with Automatic Imaging

The CellInsight family of HCS/HCA platforms offers widefield, confocal or brightfield automated imaging modes, all within a single protocol. Designed with speed in mind, real-time imaging and analysis can be run in parallel for the fastest possible turnaround time.

Publication Quality Imaging

The CellInsight family of high-content screening (HCS)/high-content analysis (HCA) platforms are designed to provide automated imaging versatility and accelerated throughput for the fastest time-to-data possible. Choose from widefield, confocal, or brightfield automated imaging modes, all within one protocol. Our instruments provide up to 12 colors to choose from, from ultraviolet through near-infrared fluorescence and multicolor brightfield imaging to enable flexible imaging paradigms. Finally, our systems are engineered with speed in mind, enabling real-time imaging and analysis conducted in parallel for the fastest time-to-data possible.   


Multicolor live-cell whole-spheroid imaging using Thermo Scientific CellInsight CX7 LZR HCS Platform in confocal mode


Onboard Real Time Analysis Software

The Thermo Scientific HCS Studio Cell Analysis Software expertly balances flexibility and ease of use to enable meaningful data analysis from single cells through populations. Parallel imaging and analysis offers true real-time results, fast. 

HCS Studio software allows backtracking of each individual cell or cellular event to minimize artifacts in the data. View the corresponding cell level cutout associated with each data point to ensure quality control of what you are actually measuring. 

Illustration showing how EurekaScan Finder software finds objects in tissue samples using 4x and 20x scanning passes

To accelerate assay development, every parameter can be evaluated for assay performance based on Z-prime statistical analysis.  This allows you to select your top-performing assays as you scale your screening campaigns. HCS Studio software also features guided workflows with user-friendly visual feedback to facilitate easy assay protocol setup, image acquisition, analysis, and scanning.


Illustration showing how EurekaScan Finder software finds spheroids and rare events using 4x and 20x scanning passes

The HCS Studio software for CellInsight CX7 LED and CellInsight CX7 LZR also feature the Thermo Scientific EurekaScan Finder software. This software significantly reduces your instrument runtime and analysis efforts by automating the detection and subsequent capture of events at higher magnification.

EurekaScan Finder software offers two different approaches based on the type of sample and user-stipulated criteria that will inform high-content screening.


Illustration showing the process of image import and conversion, insertion of data into database, and data/image export from database.

 

Specifications

Camera

Photometrics high-resolution fluorescent camera:

  • High-resolution, thermoelectrically cooled to 0°C
  • FOV 2208 x 2208-pixel array
  • 4.54 μm pixel size
  • 75% peak quantum efficiency (QE)
  • Multiple pixel binning options for flexible analysis options
Light source High-output, laser-based illumination. Configured for imaging in the UV range through near-IR range with the provided filter sets.
Objectives

Standard (Olympus™ objectives):

  • 10x (0.30 NA) Plan NeoFluar™ system
  • 20x (0.4 NA) Achroplan™ system
  • 20x (0.7 NA) Olympus objectives
  • 40x (0.6 NA) Olympus objectives

Optional (Olympus objectives):

  • 2x (0.08 NA)
  •  4x (0.16 NA)
  • 40x (0.95 NA)
  • 60x (0.90 NA)
Image compability JPG, BMP, GIF, PNG, TIF, C01, DIB
Dimensions 20 in x 32 in x 18 in (50.8 cm x 81.3 cm x 45.7 cm)
Weight 68 kg (150 lb)
Data management Compatible with Thermo Scientific Store Image and Database Management Software
Software Thermo Scientific HCS Studio 4.0 Cell Analysis Software including EurekaScan Finder double-pass functionality
Wattage 300 W

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