High accuracy PCR enzymes are used for applications requiring high accuracy during DNA amplification such as cloning, sequencing or mutagenesis. Thermo Scientific Phusion high quality DNA polymerases were created by fusing a dsDNA-binding domain to a Pyrococcus polymerase-like proofreading enzyme. Thanks to this unique protein fusion engineering, Phusion DNA Polymerases have very low error rates, fast expansion rates, and high inhibitor tolerance.

•  High fidelity—provides >100X higher sequence accuracy than Taq polymerase
  • Convenient setup—enables a universal annealing temperature of 60°C in PCR
  • Enhanced specificity—hot-start mechanism reduces non-specific amplification and primer degradation
  • Benchtop stability—automation enabled due to 24-hour stability of assembled reactions
  • High inhibitor tolerance—works with DNA of suboptimal purity

Phusion™ Plus DNA Polymerase

The new Phusion Plus reaction buffer contains proprietary additives that stabilize primer/template duplexes during the annealing step at 60°C. This unique buffer formulation helps to save time and avoid errors in PCR studies by increasing PCR specificity without the need to optimize the annealing temperature for each primer pair.
Also, different PCR reactions can be cycled together using the same protocol with Phusion Plus DNA Polymerase and universal annealing.

Phusion Plus DNA Polymerase is inert at ambient temperatures due to the hot start mechanism mediated by Affibody molecules, allowing the coupled PCR reactions to be set up and stored at room temperature. Enzyme activity is fully restored after the first denaturation step.



To simplify PCR reaction setup, Phusion Plus DNA Polymerase is also available in ready-to-use master mix formats. Phusion Plus PCR Master Mix is a convenient 2X mix of enzyme, reaction buffer, magnesium chloride and dNTPs. Only template and primers need to be added to the PCR reaction. Phusion Plus Green PCR Master Mix also contains a density reagent and two tracking dyes for direct loading of PCR products into gels.

Phusion™ Plus PCR Master Mix
Phusion™ Plus Green PCR Master Mix 

Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing. It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus PCR Master Mix to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance. Its novel reaction buffer includes proprietary additives that stabilize primer/template duplexes during the annealing step at 60°C. This unique buffer formulation increases PCR specificity without the need to optimize annealing temperature for each primer pair, helping save time and avoid mistakes in PCR runs. Furthermore, with Phusion Plus PCR Master Mix and its universal annealing feature, different PCR reactions can be cycled together using the same protocol.












Phusion Plus PCR Master Mix is inactive at ambient temperatures due to its hot-start mechanism mediated by Affibody molecules, allowing setup and storage of assembled PCR reactions at room temperature. Enzyme activity is fully restored after the initial denaturation step.

Applications

• High-fidelity PCR
• Cloning
• Template generation for sequencing
• GC-rich PCR
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High-throughput PCR

Phusion™ Plus DNA Polymerase & Phusion™ Plus Green PCR Master Mix
Amplicon size	10 kb genomic DNA, 20 kb low complexity DNA
Hot Start	Built-in Hot Start
Fidelity (vs. Taq)	>100X
For use with equipments	High Fidelity PCR
Format	2X Master Mix
GC-Rich PCR performansı	Yüksek
Overhang	Blunt
Polymerase	Phusion Plus DNA Polymerase
Reaction format	Ready to use master mix
Reaction speed	15-30 s/kb
Sufficient for	2000 reaction (50 µL)

 

Phusion™ Plus PCR Master Mix
Amplicon size	10 kb genomic DNA, 20 kb low complexity DNA
Hot Start	Built-in Hot Start
Fidelity (vs. Taq)	>100X
For use with equipments	High Fidelity PCR
Format	2X Master Mix
GC-Rich PCR performance	High
Overhang	Blunt
Polimeraz	Phusion Plus DNA Polymerase
Reaction format	Ready to use master mix
Reaction speed	15-30 s/kb
Sufficient for	500 reaction (50 µL)

Content&Storage Conditions

Phusion™ Plus DNA Polymerase

Four packs, each containing:

• Phusion Plus DNA Polymerase, 250 µL
• 5X Phusion Plus Buffer, 5 x 1.25 mL
• 5X Phusion GC Enhancer, 4 x 1.25 mL
• Store at -25°C to -15°C

Phusion™ Plus PCR Master Mix

• 2X Phusion Plus PCR Master Mix, 10 x 1.25 mL
• 5X Phusion GC Enhancer, 4 x 1.25 mL
• Water, 10 x 1.25 mL
• Store at -25°C to -15°C

Phusion™ Plus PCR Green Master Mix

Four packs, each containing:

• 2X Phusion Plus Green PCR Master Mix, 10 x 1.25 mL
• 5X Phusion GC Enhancer, 4 x 1.25 mL
• Water, 10 x 1.25 mL
• Store at -25°C to -15°C

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