Thermo Fisher Scientific

Western Blot Wet Transfer Device | Invitrogen™ SureLock™ Tandem Midi Blot Module

Designed for Western Blotting applications, the Invitrogen™ SureLock™ Tandem Midi Blot Module is a wet transfer system used only with the SureLock™ Tandem Midi Gel Tank. The tank accommodates one blotting module per room or two blotting modules in total. The Thermo Fisher Scientific™ SureLock™ Tandem Midi Blot Module is compatible with all Invitrogen™ midi gels (blot size: 8.5 cm x 14 cm).
The blotting module accommodates one midi gel per wet transfer. The unique design makes the module easy to use, while the efficient size of the inner core uses less transfer buffer per transfer (only ∼300 mL per transfer).

Since transfer buffer contains methanol, this also results in less hazardous waste requiring special disposal. At the recommended conditions and constant voltage, proteins can be transferred to nitrocellulose or PVDF membranes in 30 minutes and at room temperature.

Invitrogen™ SureLock™ Tandem Midi Blot Module

Wet Transfer Device

Features

  •  Integrative design—used with the SureLock Tandem Midi Gel Tank, eliminates the need for a separate transfer tank
    • Efficient size—transfer buffer needed is about one-third of traditional wet-transfer systems reducing methanol/transfer buffer waste and cost
    • Quick set-up (time & materials)
    • Compatible—efficiently transfers all Invitrogen pre-cast midi gels
    • Smart design—anode and cathode halves are hinged and connected by a slider. The design enables all parts to be permanently connected eliminating the need for multiple accessories.
    • Low voltage transfer (25 volts)—highly conductive anode plate separated by only 0.5 inches from the cathode plate enables efficient transfer at 25 volts within 30 minutes
    • Room temperature transfer—eliminates the need to pre-chill buffers overnight and the hassle and messiness of ice baths used with traditional wet-transfer systems that require 100 volts for transfer and generate excessive heat
    • Fast and efficient—30 minute transfer without compromising performance
    • Simple clean up—empty, rinse, and dry (no spills or ice disposal)
    • Optional SureLock Tandem Transfer Tray—can be used to set up wet transfer of midi gels using SureLock Tandem Blot Module


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Mini Blot Module

XCell II Blot Module

SureLock Tandem
Midi Blot Module

 Capacity

1 mini gel per blot module; 1-2 blot modules per tank

1-2 mini gels per blot module; 1 blot module per tank

1 midi gel per blot module, 1-2 blot modules per tank

Transfer Tank
Requirements 

220 mL per blot

800 mL

300 mL per blot

Transfer Time

60 min

60-120 min

30 min

Blot Area

9 x 9 cm

9 x 9 cm

9.2 x 14.4 cm

Compatible Power Supply

PowerEase Touch Power Supplies, Owl systems, for other systems use with Novex Power Supply Adapters (Cat. No. ZA10001)

PowerEase Touch Power Supplies, Owl systems, for other systems use with Novex Power Supply Adapters (Cat. No. ZA10001)

PowerEase Touch Power Supplies or Owl systems

Required Equipments

Mini Gel Tank

XCell SureLock Mini-Cell or
XCell II Mini-Cell

SureLock Tandem Midi Jel Tank: capacity up to 2 blot module

 

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