The unique wedge-shaped well in every Invitrogen Novex Tris-Glycine Mini Gel provides higher loading capacity, so you can load up to twice as much protein sample in every well.
 

Well Size	Recommended Loading Volume	Max. Load Volume	Max. Protein Load
10-wel	40 µL	60 µL	0.5 µg/band
12-wel	30 µL	45 µL	0.4 µg/band
15-wel	30 µL	35 µL	0.25 µg/band
17-wel	30 µL	30 µL	0.2 µg/band

Detect proteins even in dilute samples or measure expression of low-abundance proteins. With more room to load your sample, you no longer have to worry about sample spillover and cross-well contamination on your western blots. Gel loading is also easier with no need to use special gel-loading pipette tips.











Invitrogen Novex Tris-Glycine Mini Gels and Novex Tris-Glycine Plus Midi Gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis, which allows the use of Laemmli sample and running buffers. Novex Tris-Glycine Gels offer reproducible separation of a wide range of proteins into well-resolved bands.

 

Features Novex Tris-Glisin Electrophoresis Gels

• Diversity—use for native and denaturing protein assays
• Wedge-shaped wells—easily load up to two times more sample volume (Mini only)
• Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes
• Improved shelf life—store gels for up to 12 months at 4°C

Novex Tris-Glycine Gels do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using tris-glycine SDS sample buffer and a tris-glycine SDS running buffer. For native proteins, we recommend using a tris-glycine native sample buffer and a tris-glycine native running buffer.

Novex Tris-Glycine Gels use a tris-glycine discontinuous buffer system with three ions primarily involved:

• Chloride (–), supplied by the gel buffer, serves as the leading ion because it has the highest attraction to the anode relative to other anions in the system.
• Glycine (–), the primary anion provided by the running buffer, serves as the trailing ion, because it is only partially negatively charged and remains behind the more highly charged chloride ions in a charged environment.
• Tris base (+) is a common ion present in both the gel and the running buffers. During electrophoresis, the gel and buffer ions in the tris-glycine system form an operating pH of 9.5 in the separating region of the gel.

Protein Integrity

With Novex Tris-Glycine Gels, you can achieve greater protein integrity as compared to other commercially available precast gels.

 

Novex Tris-Glycine Gels offer increased protein integrity. Protein ladder, purified proteins, and E. coli lysate were loaded on a 4–20% gradient Novex Tris-Glycine Gel (A) and a supplier 4–20% gradient gel (B). Gel (B) displays numerous low molecular weight protein degradation products below major bands in lanes 3, 4, 7, 8. These are not seen in gel (A). Gel (A) also displays better lysate protein band sharpness and resolution than gel (B). Lanes 1, 10: 5 µL Mark12 Unstained Standard; lane 2: 10 µg E. coli lysate; lane 3: 6 µg catalase; lane 4: 6 µg carbonic anhydrase; lane 5: 6 µg lysozyme; lane 6: 6 µg hIgM ; lane 7: 6 µg BSA; lane 8: 6 µg beta-galactosidase; lane 9: 20 µg E. coli lysate.

Novex Tris-Glisin Gel Quality

Novex Tris-Glycine Gels are designed to deliver well-resolved, straight bands with optimal band quality as compared to other commercially available precast gels.

 

Novex Tris-Glycine gels. Protein ladders, purified proteins, and E. coli lysate were loaded on an Invitrogen Novex Tris-Glycine Gel, 4–20% gradient (A) and a supplier 4–20% gradient gel (B). Straighter lanes with better lysate protein band sharpness and resolution are observed on gel (A). Lanes 1, 5, 10: 5 µL Thermo Scientific PageRuler Unstained Protein Ladder; lanes 2, 6, 9: 5 µL Invitrogen Mark12 Unstained Standard; lane 3: 10 µg E. coli lysate; lane 4: 6 µg BSA; lane 7: 6 µg hIgG; lane 8: 20 µg E. coli lysate.

 

Novex Tris-Glycine Gels deliver sharp straight bands. Protein ladders and A431 cell lysate were loaded on a Novex Tris-Glycine Gel, 4–20% gradient and transferred to nitrocellulose using the Invitrogen iBlot 2 Gel Transfer Device. Lane 1: Invitrogen iBright Prestained Protein Ladder; Lane 2: Invitrogen MagicMark XP Western Protein Standard; Lanes 3–7: A431 cell lysate, 15 µg, 5 µg, 1.67 µg, 0.55 µg, 0.19 µg.

 

Novex Tris-Glycine Gel Features
Available Gel Sizes	Mini: 8 cm x 8 cm (1.0 mm thick) Midi: 8 cm x 13 cm (1.0 mm thick)
Storage Conditions	2–8°C
Shelf Life	Up to 12 month
Recommended Sample Buffer	SDS-PAGE: Novex Tris-Glycine SDS Sample Buffer Native-PAGE: Novex Tris-Glycine Native Sample Buffer
Recommended Runnig Buffer	SDS-PAGE: Novex Tris-Glycine SDS Running Buffer Native-PAGE: Novex Tris-Glycine Native Running Buffer
Recommended Transfer Buffer	Novex Tris-Glycine Transfer Buffer
Gel Chemistry	Tris-glycine
Available Polyacrylamide Concentrations	6%, 8%, 10%, 12%, 14%, 16%, 4–12%, 4–20%, 8–16%, 10–20%
Seperation Range (denaturation)	8–250 kDa
For Use with (Equipment) Mini Gels	Mini Jel Tank or XCell SureLock Mini-Cell
For Use with (Equipment) Midi Gels	SureLock Tandem Midi Gel Tank, Invitrogen XCell4 SureLock Midi-Cell veya Bio-Rad Criterion (only with adaptors)
Mode of Seperation	SDS-PAGE: Molecular weight Native-PAGE: Intrinsic charge, molecular size
Applications	SDS-PAGE, Native-PAGE
Well Type	Mini: 1D, WedgeWell format (load up to 60 µL per well) Midi: 1D

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