Front-end HPLC Supporting High Precision Proteome Analysis

Proteome analysis, an exhaustive analytical technique for analyzing proteins, is primarily conducted using ESI-LCMS or MALDI-TOFMS. Many proteins and peptides are extremely minute in quantity, requiring the enhanced sensitivity provided by mass spectrometers. Therefore, there is great demand for a stable front-end HPLC that can support such micro-level flow rates.

The Prominence nano is a nanoflow LC system consisting of Prominence Series units, including a flow line switching valve for nano-level flow control, and a solvent delivery unit that demonstrates excellent flow rate accuracy even at nano-level flows. Two system configurations are available, including a basic 1-dimensional system that adopts the trap injection system and a 2-dimensional system in which reversed-phase separation is automatically conducted following the first stage cation-exchange separation.

High-precision proteome analysis is strongly supported by the Prominence Series and its array of features and technologies, such as excellent retention time repeatability through a high-accuracy flow rate, a low-dead volume switching valve and its low sample carryover autosampler, to name a few.

Basic Performance of Prominence nano

• Accurate solvent delivery at nano flow rates with newly-developed nano flow sensor
• Low solvent consumption with reflux flow control system
• Low-volume nano valve – excellent performance and ruggedness –
• Higher-sensitivity analysis with less sample volume

Intuitive operation with Nano-Assist control software

• Intuitive operation via graphical user interface
• Visual monitoring of instrument operational status
• Convenient setting of 2-dimensional LC parameters

Application in Proteome Analysis

• Data reliability through high retention time repeatability
• High degree of separation utilizing 2-dimensional LC